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attune nxt acoustic focusing cytometer  (Thermo Fisher)


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    Thermo Fisher attune nxt acoustic focusing cytometer
    Attune Nxt Acoustic Focusing Cytometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/attune%C2%AE+nxt+acoustic+focusing+cytometer/attune+nxt+acoustic+focusing+cytometer/pmc12281519-85-9-14
    Average 90 stars, based on 1 article reviews
    attune nxt acoustic focusing cytometer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cytometry:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Microscopy:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Bacteria:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Probiotics:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Staining:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    FACS:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Flow Cytometry:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    SYBR Green Assay:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Concentration Assay:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Fluorescence:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.

    Incubation:

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Sperm samples were incubated with a 5 μM MitoSOXTM Red reagent working solution at 37 ◦C for 10 min. Fluorescence was measured using flow cytometry with a filter bandwidth of 574/26 nm (Attune® NxT Acoustic Focusing Cytometer, Invitrogen).

    Article Title: Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm.
    Article Snippet: Briefly, a 1x JC-1 working solution was used to stain the sperm samples for 30min before flow cytometric analysis (Attune® NxT Acoustic Focusing Cytometer, Invitrogen) with fluorescence detection at 574/26 nm (red) and 530/30 nm (green).

    Article Title: Molecular Iodine Exhibited Differential Antiproliferative Actions in Progenitor and Stem Populations from Chemoresistant Cancer Cells.
    Article Snippet: Flow Cytometry Flow cytometry was performed using the Attune® NXT acoustic focusing cytometer (Thermo Fisher Scientific, Carlsbad, CA, USA) to quantify stemness-related proteins.

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Lacticaseibacillus paracasei , Heat-killed Lacticaseibacillus paracasei lyophilized postbiotic , SYTO24 and PI as per ISO 19344 Protocol B , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Demonstrated that ISO 19344 can be adapted for postbiotics; data compared well with microscopy counts; could distinguish bacteria in product from maltodextrins. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649 , Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R , Cell cultures , LDS751 and SYTO24 , Attune® NxT Acoustic Focusing Cytometer (Thermofisher) , Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells. Method was confirmed by cell sorting. , ( ) .

    Article Title: Postbiotics: a perspective on their quantification
    Article Snippet: Microorganism(s) Product tested Staining regime Cytometer used Comments Reference Bacillus subtilis CNCM I-2745, Bacillus licheniformis NRRL B-67649, Bacillus pumilus NRRL B-67648 and BBacillus velezensis NRRL B-67647R Cell cultures LDS751 and SYTO24 Attune® NxT Acoustic Focusing Cytometer (Thermofisher) Method not specifically applied to enumeration of postbiotics, but does show promise of being able to detect non-viable spores and trace vegetative cells.



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    Flow Cytometer Attune Nxt Acoustic Focusing Cytometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/attune%C2%AE+nxt+acoustic+focusing+cytometer/attune+nxt+flow+cytometer/pm40558554-93-16-18
    Average 90 stars, based on 1 article reviews
    flow cytometer attune nxt acoustic focusing cytometer - by Bioz Stars, 2026-09
    90/100 stars
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